Title : Oxidative stress biomarkers measurement and Paraoxonase 1 status in Moroccan healthy, diabetic and hemodialysis subjects
Paraoxonase 1 (PON 1) enzyme is known to have protective effects on lipid peroxidation. Vitamin E is a chain breaking antioxidant which prevents the cyclic propagation of lipid peroxidation. The aim of the study was to investigate the PON 1 phenotype distribution and to measure oxidative stress biomarkers (Vitamin E/CT, MDA) in healthy, diabetic and hemodialysis patients.
Three hundred subjects (healthy, diabetic and chronic renal failure patients) aged between 40 and 80 years were recruited for the study. Participants were distributed in three groups of 100 subjects each. In each group, subjects were distributed in two groups, as a function of their age : middle-aged subjects, 40<age<60 years (n=147) and elderly subjects, 60≤age<80 years (n=153).
Plasma Vitamin E levels was significantly different in diabetic (p<0.01) and chronic renal failure patients (p<0.001) when compared to the healthy group. The difference in Vitamin E-Total Cholesterol ratio was also significant (p<0.001) when subjects were divided as a function of their health and age (middle-age vs elderly). Total plasma MDA concentration decreases with age in hemodialysis patients (r= ? 0.10; p=0.40) and in healthy subjects (r= ? 0.02; p=0.36). The distribution of PON 1 phenotype in healthy, diabetic and hemodialysis patients was in this order : AA > AB > BB. Adjusted odds ratio comparing the AA variant with the BB variant of PON1 were 1.97 [95 % confidence interval (CI): 0.63-6.21] in hemodialysis patients. In diabetic patients, the adjusted odds ratio comparing the AA variant with the AB variant of PON1 were 1.37 [95% CI: 0.62-3.04].
Decrease in plasma PON1 phenotype distribution may be a marker indicated the susceptibility to cardiovascular diseases complication in healthy, diabetic and hemodialysis patients.
Keywords: Vitamin E/ Total Cholesterol; MDA; PON 1; Aging; HPLC.